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Re: [visionlist] spectral sensitivity of pupil constriction in mice

Ali –

My suggestion is to use LEDs that emit around 475 nm, as we did in a study of the spectral dependence of form-deprivation myopia (Ghodsi & Stell, oral presentation at IMC 2015: attached, p. 5, abstract O13).

We used a commercially available LED array, intended for treatment of Seasonal Affective Disorder (SAD) – Philips HF3332/60 GoLITE BLU, λmax=474nm, halfwidth=25nm – which matches the peak absorption of melanopsin, in general, and specifically in mice.

This will avoid completely any illumination in the UV; and, with a long-wavelength-pass filter cutting off around 490-500 nm, you should still have adequate emission from your Ca-imaging dye in the green-yellow range, with no significant signal from the
pupilloconstricting LED.

Bill Stell

 

“Treat people as if they were what they ought to be, and you will

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– Johann Wolfgang von Goethe

 

William K. Stell, PhD, MD

Professor, Department of Cell Biology and Anatomy

 and Department of Surgery

Member, Lions Sight Centre

Member, Hotchkiss Brain Institute (HBI)

Associate Member, Alberta Children’s Hospital Research Institute (ACHRI)

University of Calgary – Cumming School of Medicine

3330 Hospital Dr. NW

Calgary, Alberta, Canada 
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From: visionlist-bounces@visionscience.com on behalf of Asli Ayaz Sent: December 22, 2016 4:27 AMTo: visionlist@visionscience.comSubject: [visionlist] spectral sensitivity of pupil constriction in mice

 

Dear all,

I am writing to get some advice on my experimental design. I would like to measure the pupil dilation/constriction as a measure of arousal in our experimental preparation ( I do 2-Photon calcium imaging of neuronal activity in somatosensory cortex of mice
performing a tactile task) For the sake of simplicity I wanted to stimulate the pupil constriction with an LED.  I know mice have two photo receptors one in UV(~360nm) range and the other M at green (~510 nm).   Since we are imaging neural activity at wavelengths
corresponding to cyan/green/yellow, I wanted to stimulate the eye at another wavelength. To make the long story short :

Is stimulating the eye at or around UV range is an option? Has anybody looked at spectral sensitivity of the pupil size of mice (my literature search failed to find one)?

My concerns/ questions are

1) 350 nm is DNA damaging wavelength (though I do not know the range of power that causes the damage)

2) Transmission of light through the lens at and near UV range is low, although reported ERG activity is still high. Would it be efficient to constrict the pupil or would I be damaging the cornea, lens ?

I can always try to shield my detection system very well and provide a visual stimuli with a screen but at the end I became curious to know the answers to the above questions. I am attaching two papers as  a reference to photo-receptor sensitivity and
the light transmission through the lens.I am looking forward to your answers and advice.

Happy Holidays

Asli

Dr. Aslı Ayaz
Brain Research Institute
University of Zurich
Winterthurerstrasse 190
8057 Zurich, Switzerland

Homepage

E-mail: ayaz@hifo.uzh.ch
Tel:  +41 44 63 53342
Fax: +41 44 63 53303
Office: Y55 H30


[visionlist] PhD studentship: Vision models and cinema postproduction

PhD studentship: Vision
models and cinema postproduction

The Information and Communication Technologies department at
Universitat Pompeu Fabra (Barcelona, Spain) invites applications for
a PhD studentship position. The doctoral student will be joining the
research group “Image Processing for Enhanced Cinematography”
(http://ip4ec.upf.edu/) and will
be co-supervised by Prof. Marcelo Bertalmío
(http://ift.tt/2hk5dEw)
and by Prof. Jesús Malo (http://ift.tt/2hyX6Qu)
from Universitat de València.

Candidates should hold an M.Sc. with a solid background in mathematics and be proficient in spoken and written English. Experience in image processing and visual perception would be highly valuable.Contact: please send application with CV, contact information of three references, and a summary of research accomplishments to marcelo DOT bertalmio AT upf DOT edu .DescriptionThe aim of the research work in this PhD Thesis is to translate motion picture know-how into better vision models, then use these models to develop image processing algorithms for cinema postproduction. As it has successfully been proven in the case of paintings, studying the craft of artists can give very profound insights into how vision works. Doing this for movies, where photographic realism can provide a very immersive surrogate for the perception of the real world, we expect to develop improved vision models for contrast and color perception that can be used to automatize filmmaking tasks, optimize results for emerging technologies, and process image content to maintain its appearance constant under changing viewing conditions. Furthermore, these models
may open up new horizons in many different fields, having important
scientific, as well as industrial and artistic, impact.

REMUNERATION:
Gross salary is 16.290 EUR the first two years,
16.850 EUR/year the third year.

Comment/web site for additional job detailsUniversitat Pompeu Fabra (UPF, http://www.upf.edu/en/)
is a public university located in Barcelona. It is the best Spanish
university according to the London Times higher education index,
2011, and it’s the number one Spanish university in number of ERC
grants. The Department of Information and Communication Technologies
(ICT, http://ift.tt/1QDiVMe)
is the best in Computer Science in Spain, according to the Shanghai
index 2009. The accreditation Units of Excellence Maria de Maeztu
2016-19 recognizes the units in Spain that perform cutting-edge
research and are among the world’s best in their respective areas:
only 3 university departments are accredited as such in Spain, and
ICT-UPF is the only one among technology and engineering.


Re: [visionlist] spectral sensitivity of pupil constriction in mice


Re: [visionlist] spectral sensitivity of pupil constriction in mice


[visionlist] PhD studentship vacancy: Goettingen, Germany


[visionlist] *Graduate Programs in Computational Neuroscience* in Berlin; MSc and PhD; deadline March 15, 2017, deadline for PhD scholarships Jan 15, 2017

[Apologies for cross-posting]

*Doctoral* and *Master Program* “Computational Neuroscience”
at the Bernstein Center for Computational Neuroscience Berlin
in Berlin, Germany

Application deadline: *March 15, 2017*
PhD Scholarships: *January 15, 2017*
Begin of courses: Sept/Oct 2017
Internet: http://ift.tt/1z3BSuL

_Doctoral Program_

The Bernstein Center for Computational Neuroscience Berlin (BCCN Berlin,
http://ift.tt/1z3BSuN) and the Research Training Group “Sensory
Computation in Neural Systems” (GRK 1589/2,
http://ift.tt/1k7kIM0) invite applications for their
PhD program.

The BCCN Berlin is a leading research center dedicated to the
theoretical study of neural processing and the *scientific program* of
the research training group combines techniques and concepts from
machine learning, computational neuroscience, and systems neurobiology
in order to specifically address ensory computation. Doctoral candidates
will work on interdisciplinary projects investigating the mechanisms of
neural computation, address the processes underlying perception on
different scales and different levels of abstraction, and develop new
theories of computation hand in hand with well-controlled experiments in
order to put functional hypotheses to the test.

The training group offers structured supervision complemented by a
teaching and training program. Each student will be supervised by two
investigators with complementary expertise and will be associated with
the Bernstein Center for Computational Neuroscience Berlin.

Candidates are expected to hold a Masters degree (or equivalent) in a
relevant subject (e.g., neuroscience, cognitive science, computer
science, physics, mathematics, etc.) and have the required advanced
mathematical background.

_Master’s Program_

The tuition-free Master program in Computational Neuroscience offers *15
places* per year, has a duration of 2 years and is fully taught in English.

The *curriculum* is subdivided into ten modules, whose content includes
theoretical neuroscience, programming, machine learning, cognitive
neuroscience, acquisition, modelling, and computational analysis of
neural data, with a strong focus on a complementary theoretical and
experimental training. Three lab rotations and a Master’s thesis are
accomplished in the second year. The aim of the program is to provide
the students with an interdisciplinary education and an early contact to
the neurocomputational research environment.

*Requirements* BSc or equivalent degree in a relevant subject (typically
in the natural sciences, in an engineering discipline, in cognitive
science, or in mathematics), certificate of English proficiency, proof
of sufficient mathematical knowledge (at least 24 ECTS credit points).

_Neurosciences in Berlin_
_International PhD Fellowships_

The BCCN Berlin is part of the newly created Einstein Center for
Neurosciences Berlin (ECN). The ECN was initiated by the joint research
networks listed below to harmonize existing training programs in the
neurosciences in Berlin, with the goal of strengthening an already
strong research landscape and consolidating a PhD education equal to the
highest international standards.

The ECN invites applications for *scholarships* for its PhD program
starting in Fall 2017. Deadline for application is *January 15* 2017.

ECN member institutions are:

The Berlin School of Mind and Brain
The Bernstein Center for Computational Neuroscience Berlin
The Center for Stroke Research Berlin
The Cluster of Excellence NeuroCure

The ECN member institutions promote cutting-edge neuroscientific
research across a wide range of different disciplines and approaches.
The ECN provides the umbrella structure that specifically fosters
interdisciplinary, collaborative research by facilitating cooperation
between these institutions, and by promoting interaction on all levels.

With around 100 internationally recognized research groups, the ECN
offers outstanding interdisciplinary training and research opportunities
for national and international scientists, with research spanning from
synapse to behavior, molecule to disease, and brain to mind.

How to apply: Visit http://ift.tt/2hvFKnl

Closing date for applications is January 15, 2017. The final interview
will take place in March 2017.

The ECN is an inter-institutional research network funded by Einstein
Foundation Berlin and jointly supported by the Charité –
Universitätsmedizin Berlin, the Freie Universität Berlin, the
Humboldt-Universität zu Berlin and the Technische Universität Berlin, as
well as the non-university partners Max Delbrück Center for Molecular
Medicine and Leibniz-Institut für Molekulare Pharmakologie.

~~~

_For more information_ …

… come and visit us at the BCCN Berlin:
http://ift.tt/2hgS52X

… or browse:
http://ift.tt/1z3BSuL

… or e-mail:
graduateprograms@bccn-berlin.de .

Best regards,

Robert Martin